Journal: Antioxidants

Article Title: Distinctive Effects of Fullerene C 60 and Fullerenol C 60 (OH) 24 Nanoparticles on Histological, Molecular and Behavioral Hallmarks of Alzheimer’s Disease in APPswe/PS1E9 Mice

doi: 10.3390/antiox14070834

Figure Lengend Snippet: Impact of fullerenol on cellular viability and reactive oxygen species (ROS) production in vitro. The percentage of viability in bEnd3, CTX TNA2, and BV2 cells is depicted in ( A ), while the level of ROS in bEnd3, CTX TNA2, and BV2 cells is shown in ( B ). Statistical significance is indicated by * p ≤ 0.05, in comparison to the control group and the group exposed solely to Aβ42.

Article Snippet: CTX TNA2 cells are type 1 astrocytes (CRL-2006, ATCC, Manassas, VA, USA), bEnd.3 cells are mouse brain endothelial cells (CRL-2299, ATCC, Manassas, VA, USA), and BV2 cells are mouse microglial cell lines (ABC-TC212S, AcceGen, Fairfield, NJ, USA).

Techniques: In Vitro, Comparison, Control

Journal: Current Issues in Molecular Biology

Article Title: Testosterone Inhibits Secretion of the Pro-Inflammatory Chemokine CXCL1 from Astrocytes

doi: 10.3390/cimb46030135

Figure Lengend Snippet: Incubation with LPC induces expression of proinflammatory genes in rat astrocytes. DI TNC1 cells were incubated for 24 h without (control) or in the presence of LPC (150 µg/mL). Subsequently, the expression of Cxcl1 ( A ) and Tnfa ( B ) genes was quantified at mRNA level by real-time PCR, and expression level was expressed relative to a validated set of reference (housekeeping) genes. Significance of overall differences was tested by one-way ANOVA ( p < 0.05). Significance of differences between treated samples and control was tested by Tukey’s post-hoc test (* p < 0.05).

Article Snippet: Type 1 astrocyte cell line DI TNC1 was purchased from ATCC (cat. no. CRL-2005).

Techniques: Incubation, Expressing, Control, Real-time Polymerase Chain Reaction

Journal: Current Issues in Molecular Biology

Article Title: Testosterone Inhibits Secretion of the Pro-Inflammatory Chemokine CXCL1 from Astrocytes

doi: 10.3390/cimb46030135

Figure Lengend Snippet: Quantification of CXCL1 secreted to culture medium by rat astrocytes. DI TNC1 cells were incubated for 24 h without (control, C), in the presence of LPC (150 µg/mL), in the presence of 60 µM testosterone (T), or in the presence of 60 µM testosterone and 60 µM flutamide (T + F). Subsequently, the amount of CXCL1 secreted by the cells was quantified in culture media by ELISA and it is presented as a value ± SEM per 100,000 cells. Significance of overall differences was tested by one-way ANOVA ( p < 0.05). Significance of differences between treated samples and the control was tested by Tukey’s post-hoc test (* p <0.001), N = 4.

Article Snippet: Type 1 astrocyte cell line DI TNC1 was purchased from ATCC (cat. no. CRL-2005).

Techniques: Incubation, Control, Enzyme-linked Immunosorbent Assay

Journal: Current Issues in Molecular Biology

Article Title: Testosterone Inhibits Secretion of the Pro-Inflammatory Chemokine CXCL1 from Astrocytes

doi: 10.3390/cimb46030135

Figure Lengend Snippet: Quantification of CXCL1 secreted to culture medium by rat astrocytes in the presence of testosterone or flutamide. DI TNC1 cells were incubated for 24 h in the presence of LPC (150 µg/mL) and in the presence of increasing concentrations of either testosterone or flutamide. Subsequently, the amount of CXCL1 secreted by the cells was quantified in culture media by ELISA, and it is presented as a value ± SEM per 100,000 cells. * p < 0.05, N = 4.

Article Snippet: Type 1 astrocyte cell line DI TNC1 was purchased from ATCC (cat. no. CRL-2005).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Current Issues in Molecular Biology

Article Title: Testosterone Inhibits Secretion of the Pro-Inflammatory Chemokine CXCL1 from Astrocytes

doi: 10.3390/cimb46030135

Figure Lengend Snippet: Incubation with testosterone induces expression of marker genes of androgen receptor in rat astrocytes. DI TNC1 cells were incubated for 24 h without (control) or in the presence of 20 µM or 60 µM testosterone. Subsequently, the expression of Fdps ( A ) and Camkk2 ( B ) genes was quantified at mRNA level by real-time PCR, and expression level was expressed relative to a validated set of reference (housekeeping) genes. Significance of overall differences was tested by one-way ANOVA ( p < 0.05). Significance of differences between treated samples, and control was tested by Tukey’s post-hoc test (* p < 0.05).

Article Snippet: Type 1 astrocyte cell line DI TNC1 was purchased from ATCC (cat. no. CRL-2005).

Techniques: Incubation, Expressing, Marker, Control, Real-time Polymerase Chain Reaction